Biology Quiz for NEET_6 (Biotechnology: Principles and Processes)

Biology Quiz for NEET_6 (Biotechnology: Principles and Processes)

1 / 30

What type of bioreactor is commonly used for the large-scale production of microorganisms?

2 / 30

Which of the following is a function of the stirrer in the bioreactor?

3 / 30

In the following figure I, II, and III are the names of the processes performed. These are respectively:

4 / 30

Which of the following product do not require a clinical trial before use?

5 / 30

In the adjacent figure, A recombinant DNA is generated. E1, E2 and E3 can be:

6 / 30

Which of the following is a probable role of divalent cation used to increase the efficiency of DNA uptake by competent cells?

7 / 30

A bacterium is engineered to produce a chemical analogue of paracetamol. Which of the following is required to make it sellable?

8 / 30

A linear DNA is treated with restriction enzymes (RE) to generate fragments of DNA as shown in the figure. If a DNA molecule is digested using only the enzyme RE2, it will produce "m" fragments. When the same DNA is digested using a combination of enzymes RE1 and RE2, it will produce "n" fragments. The value of m and n are respectively:

9 / 30

A plasmid treated with restriction enzymes (RE) to generate fragments of DNA is shown in the figure. If a DNA molecule is digested using only the enzyme RE2, it will produce "m" fragments. When the same DNA is digested using a combination of enzymes RE1 and RE2, it will produce "n" fragments. The value of m and n are respectively:

10 / 30

Which of the following can be used as a selectable marker?

11 / 30

What is the primary advantage of using PCR in recombinant DNA technology?

12 / 30

What is the primary purpose of using a vector in recombinant DNA technology?

13 / 30

Which of the following is not a step in the process of creating a recombinant DNA molecule?

14 / 30

A brief heat shock is used to enable bacteria to take up DNA from the environment. At which temperature the heat shock is given?

15 / 30

Which of the following is a correct description of the competent cell?

16 / 30

In the following table, column I contains the name of enzymes and column II contains the name of organisms from where it is isolated.

Column I Column II
i. Psp a. Pyrococcus species
ii. Pfu b. Providencia stuartii
iii. Pvu II c. Proteus vulgaris
iv. Pst I d. Pyrococcus furiosus

Which of the following is a correct match of items from column I and II?

17 / 30

In PCR melting of DNA is important for which of the following reason?

18 / 30

In PCR the extension of DNA is carried out at which temperature?

19 / 30

For the isolation of DNA by gel electrophoresis agarose is used. Agarose is a product of which of the following?

20 / 30

A retrovirus without disarming is used to transfer DNA into a host. What could be its effect on the host cell?

21 / 30

In the pBR322 plasmid, a DNA fragment is inserted at the BamH I recognition site. Which of the following is most appropriate about the bacteria carrying recombinant plasmid?

22 / 30

pBR322 is a 4361bps long plasmid and contains seven unique restriction sites. An insert of size 500bps is inserted into the tetR gene disrupting the BamH I recognition site. It is digested with four different REases EcoR I, Pvu II, Cla I and BamH I and isolated by using agarose gel electrophoresis. How many bands will be observed on the gel?

23 / 30

A plasmid named pXYZ contains restriction sites for Hind III, EcoR I, BamH I, SalI, Pvu II, Pst I, and Cla I. It is digested with a mixture of three REases, EcoR I, Pvu II, and Cla I. The approximate size of fragments generated is 2300bps, 2500bps, and 3900bps. Which of the following is correct about the size of the plasmid?

24 / 30

A plasmid named pET-31 is 4359 base pair long. An insert of size “n” bps is inserted downstream to the promoter. The insert produces a protein of size 80 residues. What is the value of “n”?

25 / 30

A retroviral genome of size 10,000kb is edited to make it a gene vector. The genes important for infectivity (gag, pol, and env) of size 5,000kb are deleted and an insert of size 0.9kb base is ligated within a gene which produces a protein of size 75 residues without disrupting the reading frame. What will be the length of polypeptide produced after the insertion of alien DNA?

26 / 30

A biosynthetic product is subjected to which of the following before it is marketed?

27 / 30

For industrial-level production of a protein, a continuous culture system is used. Which of the following is a benefit of using continuous culture?

28 / 30

The insulin gene is cloned in E. coli and made to produce insulin at a large scale. The insulin produced in bacteria is called:

29 / 30

Taq polymerase enzyme is isolated from which of the following?

30 / 30

Which of the following is a reason for not using eukaryotic DNA polymerase in PCR?

Your score is

The average score is 58%

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2 thoughts on “Biology Quiz for NEET_6 (Biotechnology: Principles and Processes)”

    1. Ritesh Kumar Yadav

      Hi I am Ritesh Kumar Yadav ,
      Yes you are right but I am not able to get 100%. But I meet in this test 80% . This test is very useful for us.
      Thank you,sir

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